Purification and Characterization of TrzF: Biuret Hydrolysis by Allophanate Hydrolase Supports Growth
نویسندگان
چکیده
منابع مشابه
Purification and characterization of allophanate hydrolase (AtzF) from Pseudomonas sp. strain ADP.
AtzF, allophanate hydrolase, is a recently discovered member of the amidase signature family that catalyzes the terminal reaction during metabolism of s-triazine ring compounds by bacteria. In the present study, the atzF gene from Pseudomonas sp. strain ADP was cloned and expressed as a His-tagged protein, and the protein was purified and characterized. AtzF had a deduced subunit molecular mass...
متن کاملStructure and function of allophanate hydrolase.
Allophanate hydrolase converts allophanate to ammonium and carbon dioxide. It is conserved in many organisms and is essential for their utilization of urea as a nitrogen source. It also has important functions in a newly discovered eukaryotic pyrimidine nucleic acid precursor degradation pathway, the yeast-hypha transition that several pathogens utilize to escape the host defense, and an s-tria...
متن کاملSequence of molecular events involved in induction of allophanate hydrolase.
Addition of urea to an uninduced culture of Saccharomyces at 22 C results in appearance of allophanate hydrolase activity after a lag of 12 min. We have previously demonstrated that both ribonucleic acid (RNA) and protein synthesis are needed for this induction to occur. To elucidate the time intervals occupied by known processes involved in induction, temperature-sensitive mutants defective in...
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15 صفحه اولHydrolysis of Fish Oil by Lipases Immobilized inside Porous Supports
A new assay was designed to measure the release of omega-3 acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid ( DHA)) from the hydrolysis of sardine oil by lipases immobilized inside porous supports. A biphasic system was used containing the fish oil dissolved in the organic phase and the immobilized lipase suspended in the aqueous phase. The assay was optimized by using a very active ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2006
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.72.4.2491-2495.2006